Because I routinely work with many-gigabyte files, looping through as mentioned in the answers didn't work for me. The solution I use:
with open(sys.argv[1], "r+", encoding = "utf-8") as file:
# Move the pointer (similar to a cursor in a text editor) to the end of the file
file.seek(0, os.SEEK_END)
# This code means the following code skips the very last character in the file -
# i.e. in the case the last line is null we delete the last line
# and the penultimate one
pos = file.tell() - 1
# Read each character in the file one at a time from the penultimate
# character going backwards, searching for a newline character
# If we find a new line, exit the search
while pos > 0 and file.read(1) != "
":
pos -= 1
file.seek(pos, os.SEEK_SET)
# So long as we're not at the start of the file, delete all the characters ahead
# of this position
if pos > 0:
file.seek(pos, os.SEEK_SET)
file.truncate()
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