Whilst you can interact with an iframe's document.styleSheets, the old-school reliable way is either to have the stylesheet there in the first place (by writing an iframe-src to point to an empty document with the desired stylesheet), or put it in place with document.write(). For example:
<body>
<iframe></iframe>
<script type="text/javascript">
var d= frames[0].document;
d.open();
d.write(
'<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.01 Transitional //EN" "http://www.w3.org/TR/html4/loose.dtd">'+
'<html><head><style type="text/css">'+
'body { font-size: 200%; }'+
'</style></head><body></body></html>'
);
d.close();
d.body.innerHTML= '<em>Hello</em>';
</script>
</body>
(This will also set the iframe document to Standards Mode, assuming that's what you want.)
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