Use the following:
Rectangle rect = new Rectangle(0, 0, 100, 100);
Bitmap bmp = new Bitmap(rect.Width, rect.Height, PixelFormat.Format32bppArgb);
Graphics g = Graphics.FromImage(bmp);
g.CopyFromScreen(rect.Left, rect.Top, 0, 0, bmp.Size, CopyPixelOperation.SourceCopy);
bmp.Save(fileName, ImageFormat.Jpeg);
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