A quick/easy method to insert date or datetime into MySQL is to use the format 'yyyy-MM-dd', or datetime as 'yyyy-MM-dd H:mm:ss'.
Try this
DateTime theDate = DateTime.Now;
theDate.ToString("yyyy-MM-dd H:mm:ss");
Make your SQL look like this.
insert into mytable (date_time_field) value ('2013-09-09 03:44:00');
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